What Is High Performance Liquid Chromatography (HPLC)?

High Performance Liquid Chromatography (HPLC) is used to separate, identify, and quantify compounds in a mixture that are dissolved in a solution. An HPLC separation involves the injection of a small volume of liquid sample into an HPLC column packed with particles (typically 3 to 5 microns in diameter) called the stationary phase. Individual components of the sample flow through the HPLC column with a liquid (mobile phase) under high pressure delivered by an HPLC pump. Typical flow rates of the liquid mobile phase used for routine analysis in QC and QA laboratories are 1–2 ml/min.

HPLC is a core technique in analytical chemistry. Routine HPLC is often used for analysis in laboratories that perform pharmaceutical drug development, quality control (QC), and quality assurance (QA), as well as food and environmental testing. Specialized LC techniques like preparative HPLC, gel permeation chromatography (GPC), or ultrahigh performance liquid chromatography (UHPLC) are also used. The choice of technique depends on the sample and amount being analyzed.

Why Is Sample Preparation Important in HPLC?

Ø Accuracy: Ensures the true representation of analyte concentration.

Ø Reproducibility: Consistent sample preparation leads to consistent results.

Ø Column Protection: Removes particulates and contaminants that can damage the column.

What Are The goals of HPLC Sample Preparation?

Ø Remove interfering species.

Ø Ensure it is the correct concentration before analysis.

Ø Release the analyte from a sample matrix.

Ø Derivatization in case sensitivity of detection requires improvement.

What Consumables Does Membrane Solutions® Manufacture in HPLC Sample Preparation?

Ø Membrane Filter

Designed for higher flow rates and larger volumes; they often have better dirt-handling capacities before clogging occurs. The raw materials membrane of PTFE, Nylon, PES, CA, MCE, RC and the different diameter from 13mm to 293mm are available.

Ø Syringe Filter

Generally effective for removing particulates from small volumes. The syringe filter PTFE, Nylon, PES, CA, MCE, RC could be provided by MS. Available in a variety of diameters and pore sizes.

Ø Sample Vial

It designed to withstand the solvents, acids, and other reagents used during sample preparation. HPLC vials often have graduated markings to allow for the measurement of small volumes, which is critical during the dilution process. Available in the cap type of 8-425 screw top, 9-425 screw top, crimp top and snap top, is compatible with Shimadzu, Agilent, Waters and JASCO mainstream instrument model.

Ø Filter Vial

It is with the functions of syringes, sample vials and disposable syringe filters, HPLC

sample preparation will be more convenient after using it.

What Are Advantages of MS® Consumables in HPLC Sample Preparation?

Ø Fast Delivery time

Ø High Cost-effective

Ø Different Series Products to Fit the Different Demand

Ø Professional Service and Supports

What Are Advantages of MS® Consumables in HPLC Sample Preparation?

Ø Research and Development Capability

All of MS membrane raw materials and products are made by ourselves, including lab, residential and industrial applications.

Ø Certificated Validation Capability

MS Validation Center is certificated as CNAS Lab.

Ø Production Capability in Raw Materials and Membrane Products

MS four factories are built for providing the membrane products with high cost-effective and faster delivery time, the production and QC control are under the ISO standards.

Ø Professional Teams

MS teams of labs, plants and sales are professional in their work and duties, all actions are promptly for the best service.

Ø Strict Management

All of MS departments work according MS strict strategies, and are trained regularly for keeping the improvement

Conclusion

Membrane Solutions® is committed to providing cutting-edge solutions that meet the evolving needs of the scientific community.

Whether you require the filtration products or professional solutions in the application of Chemical Analysis, Microbial Analysis Test, and Biological Filtration & Sterilization, please contact Membrane Solutions.

Learn more or visit our website: http://www.zsxmh.com/